Molecular Core Facility
Services
The Molecular Core Facility (MCF) at Grice Marine Lab offers molecular services to College of Charleston researchers as well as to researchers at other local institutions. We are available to provide research support through troubleshooting, optimization, data generation, and data analysis.
The MCF is here to support the needs of our scientific community and provides the following services:
- Library preparation for next generation sequencing
- Assistance with preparing samples for Sanger sequencing
- Assistance in interpreting sequencing results
- Collaboration in the design of molecular biology projects
- Design of primers for PCR, DNA sequencing, and qPCR
The Facility
The Molecular Core Facility is located in room 210 on the second floor of the Grice Marine Laboratory.
- Bioanalyzer 2100
- Thermocyclers
- Electrophoresis workstation
- Pippin Prep
- Vacuum Concentrator
- PCR Purifier Enclosure/Hood
- Microcentrifuge
- VersaDoc Imaging System
Payment
College of Charleston Users
For all CofC users, an Interdepartmental Transfer (IDT) can be completed and submitted to the lab manager and the controller’s office.
External Users
External users will receive a bill each month that should be paid within two weeks.
Payments should be made by institutional check and returned to:
The College of Charleston
Treasurer’s Office
66 George Street
Charleston, SC 29424
Please make the check payable to the College of Charleston and reference account # 350122.
You may also arrange payment by credit card using the College of Charleston’s MarketPlace (external users only).
Contact the manager of the Molecular Core Facility with any questions (hillkm1@cofc.edu).
Please contact the manager of the Molecular Core Facility to discuss your experimental design and pricing prior to submitting samples.
Fees for Services per Sample
Service | Pricing |
PCR using Promega GoTag | $0.25* |
7-well 1% agarose gel electrophoresis with ladder | $2 |
USB ExoSAP-IT PCR Purification | $1.50 |
Qiagen QIAquick Gel Extraction | $3 |
Library Preparation | Contact the MCF Lab Manager |
* researcher must provide optimized protocol and supply DNA template, PCR reagents and primers.
** researcher must provide sequencing primer(s) and a 96-well plate with lids.
DNA Library Construction for Next Generation Sequencing
Please contact the manager of the Molecular Core Facility (hillkm1@cofc.edu) to discuss your experimental design and pricing prior to submitting samples.
Laboratory Hours
The Molecular Core Facility is open from 9:00 A.M. until 4:00 P.M. Monday through Friday.
Please contact the MCF Lab Manager with any questions regarding holiday hours or to request after-hours access.
Contact
Kristina Hill-Spanik
Manager of the Molecular Core Facility
Grice Marine Lab - Room 210
College of Charleston
Phone: 843.953.9193
Fax: 843.953.9199
Email: hillkm1@cofc.edu
Additional Resources
National Center for Biotechnology Information
BOLD Identification System (COI database)