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Molecular Core Facility

Services

The Molecular Core Facility (MCF) at Grice Marine Lab offers molecular services to College of Charleston researchers as well as to researchers at other local institutions. We are available to provide research support through troubleshooting, optimization, data generation, and data analysis.

The MCF is here to support the needs of our scientific community and provides the following services:

  • DNA Sanger sequencing                                    
  • Fragment analysis (e.g., microsatellites, AFLPs)                    
  • Sample preparation guidance to obtain optimal results
  • Assistance in interpreting sequencing results            
  • Collaboration in the design of molecular biology projects           
  • Design of primers for PCR, DNA sequencing, and qPCR
  • Library preparation for next generation sequencing 

The Facility

The Molecular Core Facility is located in room 210 on the second floor of the Grice Marine Laboratory.

Molecular Core Facility

                         
Available Equipment Includes:
  • Bioanalyzer 2100                    
  • Thermocyclers                        
  • Electrophoresis workstation
  • Pippin Prep                              
  • Vacuum Concentrator              
  • PCR Purifier Enclosure/Hood
  • Beckman CEQ 8000                 
  • Microcentrifuge                        
  • VersaDoc Imaging System

Payment

College of Charleston Users

CofC users will receive an Interdepartmental Transfer (IDT) each month that should be completed and returned to the MCF Lab Manager.

External Users

External users will receive a bill each month that should be paid within two weeks.

Payments should be made by institutional check and returned to:

The College of Charleston
Treasurer’s Office
66 George Street
Charleston, SC 29424

Please make the check payable to the College of Charleston and reference account # 350122.

You may also arrange payment by credit card using the College of Charleston’s MarketPlace (external users only).

Contact the manager of the Molecular Core Facility with any questions (hillkm1@cofc.edu).

Please contact the manager of the Molecular Core Facility to discuss your experimental design and pricing prior to submitting samples.

Fees for Services per Sample

Service Pricing 
PCR using Promega  GoTag $0.25*
7-well 1% agarose gel electrophoresis with ladder $2
USB ExoSAP-IT PCR Purification $1.50
Qiagen QIAquick PCR Purification $2
Qiagen QIAquick Gel Extraction $3
DNA Sanger Sequencing $3 per sequence**
Library Preparation Contact the MCF Lab Manager

* researcher must provide optimized protocol and supply DNA template, PCR reagents and primers.

** researcher must provide sequencing primer(s) and a 96-well plate with lids.

DNA Library Construction for Next Generation Sequencing

Please contact the manager of the Molecular Core Facility (hillkm1@cofc.edu) to discuss your experimental design and pricing prior to submitting samples. 

Laboratory Hours

The Molecular Core Facility is open from 9:00 A.M. until 4:00 P.M. Monday through Friday.

Please contact the MCF Lab Manager with any questions regarding holiday hours or to request after-hours access.

Contact

Kristina Hill-Spanik
Manager of the Molecular Core Facility
Grice Marine Lab - Room 210
College of Charleston
Phone: 843.953.9193
Fax: 843.953.9199
Email: hillkm1@cofc.edu

Additional Resources

National Center for Biotechnology Information

BOLD Identification System (COI database)